ABSTRACT
Insulin-like growth factor II [IGF-II] is a crucial factor in regulating cell proliferation, growth, migratioi differentiation and survival. The objective of this study was to investigate the association of polymorphisi in intron 3 and exon 3 of insulin like growth factor II [IGF-Il] gene and body weight trait in tilapia nilotic fish using restriction fragment length polymorphism [RFLP] and single nucleotide polymorphism [SN1 techniques. Thirty tilapia nilotica fish were precisely selected according to their body weight and ordere from the largest to the smallest body size. DNA was extracted from fish blood samples to amplify 397-bp of IGF-II gene. Amplified IGF-II gene was sequenced only in the seven large and five small size fish. Resul revealed that restriction analysis [RFLP] with MSP of the [IGF-II] gene [397-bp] do not produ restriction fragments. While, DNA sequencing showed fourteen single nucleotide polymorphisms [SNP' at different positions in large size fish as they were detected in more than one fish at nucleotides numb* 318 [C-"T], 319 [A->T], 320 [G-"A in three fish], 322 [C-"A and C-"G], 323 [G->C and G-"A], 32 [G-"A in three fish], 332 [A-"T in three fish], 333 [C-"A and C-"T], 334 [A-"C in two fish], 337 [C-" in two fish] and 340 [C-"T]. Hence, our results reveal the possibility to use these findings as marker for selection of high body weight trait in tilapia nilotica fish
ABSTRACT
Nucleotide polymorphism [SNP] techniques were used to study the associate polymorphism in exon 1 of growth hormone receptor [GHR] gene and marketing body weights in three rabbit breeds: New Zealand White rabbit breed [pure breed], V-line [Hybrid breeds from New Zealand and California rabbit], and Alexandria breed [Hybrid from V-Line and black Egyptian Baladi rabbit]. DNA from blood samples of each kit of the three rabbit breeds were extracted to amplify 263-bp of the gene encoding GHRl. 1 he purified JCR products were sequenced in those had the highest and lowest body weight in each breed. Sixteen new SNPs were detected in GHRl gene in New Zealand rabbits. In V-line rabbits, three SNPs were detected. Also two SNPs were found in Alex. breed. All these SNPs were related to high body weight as they were reported in higher body weights in the three breeds, and were non synonymous, which led to amino acids substitution. Restriction analysis of PCR product using MSP1 do not produce restriction fragments. Statistically, the results indicated that there were significant correlation between growth performance and GHRl gene polymorphisms. The results were effective in selection for rabbits that have high growth performance [marker assisted selection]. Also, the analysis of this study confirmed that GHRl gene could be a candidate gene for application in marker assisted selection [MAS] in rabbits
ABSTRACT
Genotoxic effect of acute and chronic doses of Bentazon and Glyphosate herbicides were estimated using micronucleus [MN], nuclear budding, mitotic index [MI] and chromosomal aberration assays. Five groups of Swiss male albino mice were used in acute exposure of 1/5 and 1/10 LD50 of both herbicides after 24, 96 hours of exposure compared to the control. For chronic exposure 1/20 of LD50 of each herbicide was for two months. Genotoxicity of acute doses at different duration of Bentazon and Glyphosate were confirmed through significant increased number of aberrant cells, different types of chromosomal aberration, micronucleus and nuclear budding. Chronic dose of Bentazone induced more genotoxic effects than Glyphosate